Cell transfection is an essential step for gene editing and intracellular delivery of cargoes such as mRNA and proteins. Significant improvements have been made to reduce the cytotoxicity and to improve efficiency associated with transfection over the few decades. Nevertheless, with new, exciting biological questions, the demand for the ideal transfection technique with high throughput, single cell transfection ability and control of cargo dosage has increased. This review focuses on recent innovations in cell transfection techniques, and discusses the pros and cons of each method.