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Joshua Hill
Texas A&M AgriLife Genomics and Bioinformatics Service, 2123 TAMU, College Station, TX 77843, USA
joshuaehill@gmail.com
Wery’s et al. recent paper “Zincmediated RNA Fragmentation allows robust transcript reassembly upon whole genome transcriptome RNA-Seq” sheds light on a problem that affects many Next Generation Sequencing (NGS) experiments; the ability to accurately represent the RNA present in a given
sample. Bias for RNA-seq can be simply defined as a false increase or decrease of a given RNA transcript. If that definition is expanded over a whole
transcriptome then it is easy to see how false conclusions can be made and why bias must be considered......
 
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