I am interested in dissecting the functions of Tet1 in mouse embryonic development. Tet1 is a member of the Tet family of enzymes that catalyse the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), a hallmark of the pluripotent state in embryonic stem cells (ESCs).  In addition to the Tet catalytic functions, Tet1 has catalytically independent roles in ESCs; the majority of which are mediated via the interaction with other epigenetic modifiers, like Hdac1, post-translational modifiers, such as Ogt1 and Parp1, as well as repressor complexes, PRC2 and Sin3A. As such, I study Tet1-catalytically mutant and Tet1-depleted cell lines and mice to elucidate the precise roles of Tet1, eventually contributing to the generation of better induced pluripotency.

1.    A Critical Role of TET1/2 Proteins in Cell-Cycle Progression of Trophoblast Stem Cells.

Chrysanthou S, Senner CE, Woods L, Fineberg E, Okkenhaug H, Burge S, Perez-Garcia V, Hemberger M. Stem Cell Reports. 2018 Apr 10;10(4):1355-1368. doi: 10.1016/j.stemcr.2018.02.014.Epub 2018 Mar 22.

2.    ADP-ribosyltransferases Parp1 and Parp7 safeguard pluripotency of ES cells.

Roper SJ, Chrysanthou S, Senner CE, Sienerth A, Gnan S, Murray A, Masutani M, Latos P, Hemberger M. Nucleic Acids Res. 2014Aug;42(14):8914-27. doi: 10.1093/nar/gku591. Epub 2014 Jul 17.

3.    Genetic polymorphisms in warfarin and tacrolimus-related genes VKORC1, CYP2C9 and CYP3A5 in the Greek-Cypriot population.

Hadjipanagi D, Chrysanthou S, Voskarides K, Deltas C. BMC Res Notes. 2014 Mar 5;7:123. doi: 10.1186/1756-0500-7-123.